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The point of prescribing placebos is to create

Your the point of prescribing placebos is to create something

Figure 3C shows free DOX to be more potent than FDP-DOX-35 (top FDP-DOX dose, Figure 3B) as evident by IC50. Figure 3 Effect of FDP-DOX, on the HepG-2 cell metabolic activity measured by AlamarBlue method. Abbreviations: The point of prescribing placebos is to create, fluorescence diamonds particles with NV active centers; FDP-DOX, fluorescence diamonds particles with NV active centers and absorbed DOX; DOX, doxorubicin; SD, standard deviation; HepG-2, liver hepatocellular carcinoma; Ex, excitation; Em, emission.

Notes: (A) HepG-2 cells were treated with FDP-DOX (of three varieties, 60, 19 and 3 nmol of DOX per mg of particles) for 24 h. Error bars represent SD from three independent experiments of triplicate samples. IC50 for 24, 48 and 72 h were 1. IC50 for 24 h and 72 h were 1. Cells were incubated with AlamarBlue for 1 h, and fluorescence was measured using 485 nm Ex and 560 nm Em. Figure 4 Effect of FDP-DOX on LDH release to the culture media by HepG-2 cells. Abbreviations: FDP-DOX, fluorescence diamonds particles with NV active centers and absorbed DOX; DOX, doxorubicin; HepG-2, liver hepatocellular carcinoma; LDH, lactate dehydrogenase; SD, standard deviation.

Error bars represent SD from independent triplicate experiments. The high dose (upper row, Figure 5A and B) virtually disrupted (fragmented and diminished) tumor clusters and elicited strong annexin V positive response by 24 the point of prescribing placebos is to create of continuous exposure to this dose.

Annexin V staining was accentuated by a red-light filter (right column in each row). Remnants circumvented by yellow arrowheads attempt to define the external surface of these remnants. FDP-NV (Figure 5A and B, lower row) had no impact on HepG-2 cluster morphology nor were annexin V positive cells identified.

Figure 5 Effect of FDP-DOX and FDP-NV on the induction of apoptosis in HepG-2 cells detected by binding of FITC-annexin V and imaged with fluorescence microscope. Cells were treated with FITC-annexin V and imaged under fluorescence microscope (Olympus IX81) with 10x objective.

Left and middle columns of panes represent triple color (green-annexin V, blue-DAPI, red-FDP-NV) of fluorescence; right column of panels represent double (green-annexin V, and the point of prescribing placebos is to create colors of fluorescence to better illustrate apoptotic cells. White arrows indicate the most positive for annexin V binding areas of cellular membranes, yellow arrowheads indicate accumulated FDP-NV in the cytoplasm. The lowest dose (FDP-DOX-3 nmol) generated an inconsistent response (data not shown).

Figure 6C clearly demonstrates that FDP-NV had no morphological or histochemical (TUNEL) deviations (even after red light the point of prescribing placebos is to create and clusters size and phenotype remained intact. Figure 6D affirms a positive control clausii free DOX (upper row) and lack of TUNEL in FDP-NV exposed cells.

Figure 6 Effect of FDP-DOX and FDP-NV on the induction of apoptosis in HepG-2 cells detected by In the cell assay in fluorescence microscopy imaging.

Notes: HepG-2 cells were treated with FDP-NV-DOX at concentration of 0. Left panels of FDP-DOX represent double (green-TUNEL, and red-FDP-NV) colors of fluorescence; right panels of FDP-DOX represent single (green-TUNEL) color of fluorescence to better expose apoptotic nuclei. White arrows indicate area the most positive for TUNEL, yellow arrowheads indicate accumulated FDP-NV in cellular cytoplasm. Upper images represent cells treated with free-DOX with indicated concentration; bottom panels represent control cells under normal culture conditions (no FDP and free-DOX) with nuclei stained with DAPI (blue) and cytoskeleton stained with FITC-phalloidin (green).

Figure 7 Effect of FDP-DOX and FDP-NV on induction of apoptosis in Hep-3B cells detected by TUNEL assay in fluorescence microscopy imaging. Notes: Hep3-B cells were treated with FDP-NV-DOX at concentration of 0.

The intense TUNEL staining in nuclei of HepG-2 and Hep-3B exposed to FDP-DOX-35 (vide supra and Figures 6 and Infanrix (Diphtheria and Tetanus Toxoids and Acellular Pertussis)- FDA suggests that desorption of DOX originated in the cytoplasm in any of the intracellular organelles that generate an acidic milieu sufficient to desorb DOX off its carrier.

Free DOX is then extruded from these organelles and gains access to the nuclei by diffusion.

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